Acta Scientiarum Polonorum
Silvarum Colendarum Ratio et Industria Lignaria

The research was carried out at the Trzciel Breeding Center in the Trzciel Forest District. Samples were collected at random from hunted roe deer during the 2016/17 hunting season. Blood samples of 19 killed animals were collected, labeled and stored at –18°C. The pathogens were detected with the use of PCR and nested PCR methods. To detect T. gondii in animal blood and ticks the two pairs of primers specific to the B1 gene were used. The presence of B. burgdorferi s.l. was detected with the use of a pair of primers specific to the flagelline gene, while two pairs of primers specific to the gene coding 16S rRNA were used to detect A. phagocytophilum. B. microti, on the other hand, was detected with the use of two pairs of primers specific for the 18S rRNA gene. The presence of the reaction products with the size of base pairs [bp] 531 bp for T. gondii, 482 for B. burgdorferi s.l., 932 bp and 546 bp for A. phagocytophilum, 238 bp and 154 and for B. microti were treated as positives. Neither T. gondii nor B. burgdorferi s.l. were detected in any samples, A. phagocytophilum was present in two samples (10.2%) and B. microti was present in seven (36.8%). T. gondii was present in 2% of Dermacentor reticulatus ticks.